Phospholipid-analogous compounds

ABSTRACT

Phospholipid-analogous compounds of the general formula (I)                    
     in which A                    
     where 
     R 1  and R 2  are, independently of one another, hydrogen, a saturated or unsaturated acyl or alkyl radical which can optionally be branched or/and substituted, where the total of the carbon atoms in the acyl and alkyl is 16 to 44 C atoms, 
     s is an integer from 0 to 8, 
     c is a radical of a primary or secondary alcohol of the formula RO—, where R is a saturated or unsaturated alkyl radical, mainly with cis double bond, of from 12 to 30 carbon atoms, 
     n is an integer from 2 to 8, 
     R 3    
     a can be 1,2-dihydroxypropyl or 
     b can be alkyl with 1 to 3 C atoms when z is&gt;0 or 
     c can be alkyl with 1 to 3 carbon atoms when n≠2 and z=0, 
     m is 1 or 2, 
     x is an integer from 0 to 8, 
     y is 1 for z=1 to 5 or 
     is 1 to 4 for z=1 
     z is an integer from 0 to 5, 
     are novel and are suitable as liposome constituents, solubilizers and pharmaceuticals.

This is a Division of application Ser. No. 09/486,039 filed Feb. 18, 2000 now U.S. Pat. No. 6,334,576. The disclosure of the prior application is hereby incorporated by reference herein in its entirety.

DESCRIPTION

The invention relates to novel phospholipid-analogous compounds which can be employed as liposome constituents for transporting pharmaceuticals, as solubilizers for pharmaceuticals of low solubility in water and also as active ingredients themselves for disorders such as cancer and leishmaniosis, to liposomes containing these novel compounds, to pharmaceutical compositions which contain these liposomes, and to processes for producing pharmaceuticals.

The invention relates in particular to phosphatidyl compounds which contain a defined hydropholic radical, and to liposomes which have a shortened or lengthened lifespan in the serum and can be taken up specifically by particular cells, for example tumour cells.

The retention time of conventional liposomes in the serum is up to 5 hours. However, it is desirable, especially when liposomes are used as carriers of active pharmaceutical ingredients, for the liposome retention time in the blood circulation to be as long as possible, but especially in conjunction with uptake in selected target cells.

Hence the so-called stealth liposomes which have a lengthened lifespan were developed. These stealth liposomes have a structure based on phosphatidyl compounds which contain an extended polyethylene glycol residue. The polyethylene glycol residue proved to be most effective for the desired lengthened lifespan with molecular weights between 2000 and 3000. A considerable disadvantage of these stealth liposomes and of the phosphatidyl compounds with polyethylene glycol residue is, however, that they are not accurately defined compounds because the polyethylene glycol residues have different chain lengths. However, the so-called stealth liposomes always have, as a consequence of the phosphate radical, a negative surface charge in the liposome membrane. The object of our own earlier patent application 196 22 224.9 was therefore to provide compounds which increase the lifespan of liposomes and have a composition which can be stated accurately, likewise using phosphate esters and thus negative charges.

An object of the present invention is, by contrast, to provide compounds which avoid this negative surface charge and link the oligoglycerol or sugar residues by a nitrogen atom into the structure. The positive charge on the nitrogen residue balances the negative charge on the phosphate, or even overcompensates when 2 nitrogen atoms are used in the molecule. This object is achieved according to the invention by a compound of a general formula I

in which A

where

R₁ and R₂ are, independently of one another, hydrogen, a saturated or unsaturated acyl or alkyl radical which can optionally be branched or/and substituted, where the total of the carbon atoms in the acyl and alkyl is 16 to 44 C atoms,

s is an integer from 0 to 8,

c is a radical of a primary or secondary alcohol of the formula RO—, where R is a saturated or unsaturated alkyl radical, mainly with cis double bond, of from 12 to 30 carbon atoms,

n is an integer from 2 to 8,

R₃

a can be 1,2-dihydroxypropyl or

b can be alkyl with 1 to 3 C atoms when z is>0 or

c can be alkyl with 1 to 3 carbon atoms when n≠2 and z=0

m is 1 or 2,

x is an integer from 0 to 8,

y is 1 for z=1 to 5 or

is 1 to 4 for z=1

z is an integer from 0 to 5.

Preferred compounds are those where

m=1, n=2, x=0, z≠0, and R₃ is an alkyl radical, do not apply simultaneously.

The structural elements used in the substances described herein can be varied at will and adapted to suit the particular use.

It is possible via structural parameter A to vary mainly the apolar portions of the molecules, for example via the chain length of the fatty acids and of the alkyl radicals. A modification of the polar portions is possible via the phosphate group, the nitrogen atom and the oligoglycerols linked thereto.

The compounds embraced by the general formula I have excellent biological properties and are used as

1) liposome constituents for specific accumulation of active ingredients in target cells,

2) solubilizers for substances which are difficult to administer intravenously such as, for example, Taxol,

3) active ingredients for cancer and protozoal diseases.

The compounds which have particular importance for the various applications are now described in detail. There are overlaps in this because disubstituted glycerols with the structural feature A may have both membrane-stabilizing properties (R₁+R₂>20 C atoms) and membrane-destabilizing properties (R₁+R₂<20 C atoms). In particular, the boundary regions between membrane- and micelle-formers may be of special interest here.

It is common to all the structures that the novel molecules are simple to prepare, this being possible by reacting primary or secondary amines with epoxides. Thus, 1,2-dipalmitoyl-sn-glycero-3-phospho(N-methyl)ethanolamine with benzylglycidol results, after catalytic debenzylation and methylation on nitrogen, in a phospholipid with a lecithin-like structure which is used as liposome constituent.

Compounds with only one long alkyl or acyl chain have other interesting properties if they are linked via primary or secondary amines to epoxides, as is evident from the description hereinafter. They are excellent solubilizers for active ingredients which are difficult to administer intravenously and are in fact direct active ingredients for cancer and leishmaniasis.

The stepwise assembly, on which this invention is based, of the hydrophilic radicals of the phosphatidyl compounds of the formula I makes it possible to take an accurately defined composition of the compounds.

Thus the compound of the formula I according to the invention does not comprise a mixture of different molecules of indefinite composition and chain length; on the contrary, it is possible to obtain specifically a desired structure. This means that, if the desired product is an N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxypropyl)ammonium derivative with y=1 and z=2 in formula I, the compound is chemically defined and contains no portions with y=1 and z=1 or y=1 and z=3 etc. Hydroxypropyl derivatives having a wholly defined chain length and essentially free of other chain lengths are preferably used.

The compound of the formula I is, according to the invention, a homogeneous compound of defined structure. The compound is preferably more than 99% homogeneous in relation to the value of z. However, it is also possible to provide the compound with a homogeneity of more than 99.9% in relation to the value of z.

The compound preferably comprises hydroxypropyl derivatives on the nitrogen with 1 to 5 hydroxypropyl units, preferably with 1 to 3 hydroxypropyl units. Preferred in this connection are 1,3-linked linear oligoglycerol residues which are linked via a 2-hydroxypropyl radical to the nitrogen atom.

The radical for A=c with the formula RO— is derived from the primary or secondary alcohol. When RO— is derived from a secondary alcohol, radicals with the oxygen on the C₂, C₃ or C₄ atom are preferred.

The radicals R¹ and R² are according to the invention preferably independently of one another hydrogen, a saturated or unsaturated acyl or alkyl radical which may optionally be branched or/and substituted, where the total of the carbon atoms in the acyl and alkyl is between 16 and 44.

The invention further relates to liposomes which contain phospholipids or/and alkylphospholipids, where appropriate cholesterol and 1 to 50 mol % of a compound of the general formula I.

The phospholipids or/and alkylphospholipids can be, for example, diacylglycerophospho compounds of defined structure. It is generally possible to employ these constituents of the lipids as compounds of defined structure.

In the case where y>1, the radical —CH₂(CHOH)_(y)—CH₂—OH is preferably derived from sugar alcohols which have 3 hydroxyl group for y=2, 4 hydroxyl groups for y=3 and 5 hydroxyl group for y=4. Examples of such radicals are mannitol derivatives for y=4, lyxitol derivatives for y=3 and threitol derivatives for y=2.

The liposomes according to the invention have a distinctly modified half-life in the blood circulation. The liposomes with m=1 are neutral to the outside and show increased retention times in the blood, while the liposomes with m=2 circulate for only a very short time as a consequence of the excess positive charge in the membrane.

The invention further relates to a pharmaceutical composition which contains the liposomes described above and one or more active pharmaceutical ingredients entrapped in the liposomes, where appropriate together with pharmaceutically customary diluents, excipients, carriers and bulking agents.

It is possible as a rule to use as active ingredients all active ingredients which can in fact be introduced into the plasma by means of liposomes. Preferred groups of active ingredients are, on the one hand, cytostatics, in particular anthracycline antibiotics such as, for example, doxorubicin, epirubicin or daunomycin, with doxorubicin being particularly preferred. Further preferred cytostatics are idarubicin, hexadecylphosphocholine, 1-octadecyl-2-methyl-rac-glycero-3-phosphocholine, 5-fluorouracil, cis-platinum complexes such as carboplatin and Novantrone, and mitomycins.

Further preferred groups of active ingredients are immunomodulating substances such as, for example cytokines, with particular preference being given among these in turn to the interferons and, in particular, α-interferon, substances with antimycotic activity (for example amphotericin B) and active ingredients for protozoal diseases (malaria, tyrpanosomal and leishmania infections). Taxol is likewise preferred as active ingredient.

Another preferred group of active ingredients are lytic active ingredients like those described in DE 41 32 345 A1. Preference is given to miltefosin, edelfosin, ilmofosin and SR162-834. Particular preference is given to alkyphosphocholines also with extended alkyl chains, for example erucylphosphocholine and erucylphosphocholines with increased phosphonitrogen distance.

The present invention further relates to the use of liposomes according to the invention for producing an antitumour composition, in which case the active ingredient is particularly preferably doxorubicin.

The present invention furthermore relates to the use of the liposomes according to the invention for producing a composition for influencing cell proliferation, in which case the active ingredient is a cytokine, particularly preferably α-interferon.

The liposomes according to the invention are prepared by methods known per se using apparatus customary for this purpose. It is possible typically to convert a solution containing the various components of the liposome, including 1 to 50 mol % of a compound of the formula I, into a lipid suspension which is then forced under high pressure through nozzles or through perforated plates, it being possible to control the size of the resulting liposomes by the size of the orifices in the perforated plate. Suitable measures for converting a lipid suspension into liposomes are known to the skilled person. Preferably, 5 to 55 mol % of a compound of the general formula I are converted with 35 to 60 mol % of cholesterol and 40 to 60 mol % of phospholipids or/and alkylphospholipids into a lipid suspension which is then converted by suitable measures in a manner known per se into liposomes. Such known processes can also be used to produce a pharmaceutical preparation which contains the liposomes according to the invention and one or more active pharmaceutical ingredients. For entrapping water-insoluble active ingredients, the active ingredient is dissolved together with the lipid constituents while, for entrapping water-soluble active ingredients, an aqueous solution containing the water-soluble active ingredient is added to the lipid film.

The initial phospholipids are produced by processes described in the literature (DE 31 30 867 A1: Eibl et al., Chem. Phys. Lipids 28 (1981)), 1-4 41 (1986), 53-63 and 47 (1988), 47-53. It is possible here in particular to have recourse to processes described in the PCT/EP97/00749 application of Feb. 17, 1997. Thus, the compounds according to the invention of the formula I can be prepared in the following way:

EXAMPLE A=a; n=2; m=1; y=1; z=2

(Reaction of 1,2-dipalmitoyl-sn-glycero-3-phospho(N-methyl)ethanolamine with 1,2-isopropylidene-glyceroglycidol)

Corresponding reactions can be carried out to afford compounds with n=3-10, m=1, y=1, z=1-5. Starting compounds for x=2 and 3 are cephalins and N-methylcephalins, whose preparation has been described in detail. The reactions with the corresponding glycidols, whose preparation is described in the German patent application “Phosphatidyloligoglycerine” 19622224, leads to the required products, the addition being carried out in a 2-phase system of THF-Na₂CO₃/NaHCO₃ 1:1 (0.5 M in H₂O; pH 9-10). However, no hydrolysis of the fatty acid esters is observed at these pH values.

To prepare the dioleoyl compounds it is necessary to prepare 1,2-dioleoyl-sn-glycero-3-phospho-(N-methyl)ethanolamine. This is achieved by the following reaction scheme:

Unsaturated compounds are generally prepared analogously.

It is possible correspondingly to prepare compounds with n=4-8 because the corresponding terminal alkanolamines can be purchased and can be converted into the N-BOC-protected compounds.

Examples of a synthesis of liposome constituents are described below to demonstrate the experimental breadth of the present application. It is possible, as is evident from the examples, to prepare any fatty acid ester and alkyl ether combinations which vary in chain length, number of cis double bonds and degree of branching.

The RF values of the exemplary compounds 1 to 279 were determined in the system CHCl₃/CH₃OH/glacial acetic acid/H₂O=100/60/20/5 parts by volume. They are grouped very close together, specifically as follows:

RF Compound No. 0.10-0.15 129-135 0.15-0.20 117-128, 167, 172, 209-216 0.20-0.25 70-84, 98-116, 151-166, 191-208 0.25-0.30 45-69, 136-150, 183-196, 262-272 0.30-0.35 25-44, 173-182, 255-261, 273-279 0.35-0.40 1-24 0.40-0.45 85-97 0.30-0.40 217-240 0.20-0.30 241-254

EXAMPLES

A) Preparation of erucoyl-1,3-propanediol-phospho-N,N,N-trimethylpropylammonium (Example 176)

Erucoyl-1,3-propanediol

1,3-Propanediol, 153 g (MW 76.1; 2 mol) is dissolved in 1 l of THF and, after addition of 60 g of triethylamine (MW 101.2, 0.6 mol) and 7.3 g of 4-dimethylaminopyridine (MW 122.2, 0.06 mol), equilibrated at 20° C. in a water bath. While stirring continuously, 178 g of erucoyl chloride (MW 357.0; 0.5 mol) in 500 ml of THF are slowly added dropwise so that the temperature in the reaction mixture does not exceed 30° C. The dropwise addition is followed by warming at 30° C. for 30 minutes and then addition of 1.5 l of diisopropyl ether and 1.5 l of 1N HCl. After vigorous shaking and phase separation, the upper ether phase is washed once again with 1% NaCl solution and evaporated in vacuo at 45° C. The residue is taken up in 2 l of hexane and cooled to −20° C. The white crystals are filtered off with suction and dried in vacuo. The yield of pure erucoyl-1,3-propanediol is 123 g (MW 396.7; 62%). The substance is pure by thin-layer chromatography (Rf value 0.3 in ether/pentane/acetic acid 200/200/2 parts by volume).

Microanalysis

C₂₉H₄₅O₃ calc.: C, 75.70; H, 12.20; O, 12.10; found: C, 75.81, H, 12.16, =,-.

Erucoyl-1,3-propanediol-phospho-N-methylpropylammonium

Phosphorus oxychloride, 24.2 g (MW 153.33; 0.16 mol) in 15 ml of THF is cooled to 0° C. in an ice bath. A solution of erucoyl-1,3-propanediol, 60 g (MW 396.7; 0.15 mol) and 17.2 g of triethylamine (MW 101.19; 0.17 mol) in 250 ml of THF is added dropwise with stirring so that the temperature in the reaction mixture does not exceed 15° C. After the dropwise addition of the solution, the temperature of the reaction mixture is brought to 20° C. and stirred for a further 30 minutes.

The conversion of the formed erucoyl-1,3-propanediol-phosphoric acid dichloride into the target product takes place by reaction with N-methylpropanolamine via an intermediate six-membered ring. This is done by adding a solution of 16 g of N-methylpropanolamine (MW 89.14; 0.18 mol) and 35.4 g of triethylamine (MW 101.19; 0.35 mol) in 250 ml of THF dropwise to the stirred reaction mixture in such a way that the temperature does not exceed 35° C. After the dropwise addition, the reaction mixture is kept at 25° C. for 30 minutes. The precipitated triethylamine hydrochloride is filtered off. The filtrate contains the intermediate (Rf 0.25 in CHCl₃/ethyl acetate 1:1, parts by volume) and is converted by adding 60 ml of 2 N HCl with ring opening into erucoyl-1,3-propanediol-phospho-N-methylpropylammonium (Rf 0.45 in CHCl₃/CH₃OH/glacial acetic acid/H₂O 100/80/10/5, parts by volume). The THF solution of the product is mixed with 200 ml of 0.2 M sodium phosphate solution (pH˜8.0) and adjusted to pH 6-7. The product is precipitated by adding 1 l of acetone, and the crystals are isolated by filtration with suction at 4° C. Erucoyl-1,3-propanediol-phospho-N-methylpropylammonium is sometimes slightly impure. Chromatography on silica gel with CHCl₃/CH₃OH/H₂O can be employed for purification. The yield of pure product—based on erucoyl-1,3-propanediol—is 65 g (MW 547.8; 79%).

Microanalysis

C₂₉H₅₈NO₈P calc.: C, 63.59; H, 10.67; N, 2.56; O, 17.53; P, 5.66; found: C, 63.34; H, 10.49; N, 2.49; O, -; P, 5.59.

Erucoyl-1,3-propanediol-phospho-N,N,N-trimethylpropylammonium

Erucoyl-1,3-propanediol-phospho-N-methylpropylamine, 54.8 g (MW 547.8, 0.1 mol) is mixed with 800 ml of THF and 83 g of K₂CO₃ (0.6 mol) in 800 ml of H₂O. The mixture is heated to 50° C. to result in a two-phase solution. While stirring vigorously, 74.5 g of methyl toluenesulphonate (MW 186.23; 0.4 mol) in 200 ml of THF are added dropwise, and the mixture is boiled under reflux. The reaction is complete after 60 minutes. Erucoyl-1,3-propanediol-phospho-N,N,N-trimethylpropylammonium is precipitated as product (Rf value 0.1 in CHCl₃/CH₃OH/glacial acetic acid/H₂O 100/80/10/5, parts by volume) from the THF phase with 1.2 l of acetone, and the precipitate is taken up in 300 ml of CHCl₃, filtered and again precipitated with 1.2 l of acetone. If the product is not quite pure, chromatography on silica gel with CHCl₃/CH₃OH/H₂O can be employed for purification. The yield of pure product—based on the N-methyl compound—is 49 g (MW 575.8; 85%).

Microanalysis

C₃₁H₆₂NO₆P calc.: C, 64.66; H, 10.85; N, 2.43; O, 16.67; P, 5.38; found: C, 64.38; H, 10.81; N, 2.39; O, -; P, 5.27.

B) Preparation of erucoyl-1,3-propanediol-phospho-N,N-dimethyl-N-(hydroxypropyldihydroxypropyl)propylammonium (Example 155)

This compound can be prepared by using the intermediate erucoyl-1,3-propanediol-phospho-N-methylpropylammonium. It is converted in a reaction firstly with an epoxide and immediately further methylated to give the final product. Erucoyl-1,3-propanediol-phospho-N-methylpropylammonium, 54.8 g (MW 547.8; 0.1 mol) is mixed with 800 ml of THF and 83 g of K₂CO₃ (0.6 mol) in 800 ml of H₂O and heated to 50° C. to result in a two phase solution. While stirring vigorously, a solution of 21 g of 1,2-isopropylideneglycero-3,1-glycidol (MW 188.2; 0.11 mol) in 200 ml of THF is added dropwise, and the temperature is raised to 60° C. The reaction mixture is boiled under gentle reflux for 2 hours, and 37 g of methyl toluenesulphonate (MW 196.23; 0.2 mol) in 100 ml of THF are added. The reaction is complete after boiling under reflux for 2 hours. The THF phase is substantially evaporated at 45° C., and the residue is heated with 300 ml of 70% acetic acid at 60-65° C. to remove the isopropylidene protective group. A mixture of 1 l of CHCl₃, 1 l of CH₃OH and 1 l of 1% strength NaCl solution is added to the reaction mixture and, after thorough shaking, the solvent is removed from the lower CHCl₃ phase in vacuo. The residue is taken up in 400 ml of CHCl₃ and precipitated with 1.6 l of acetone. Chromatography on silica gel with CHCl₃/CH₃OH/H₂O can be employed for purification. 46 g of erucoyl-1,3-propanediol-phospho-N,N-dimethyl-N-(hydroxy-3,1-dihydroxypropyl)propylammonium (MW 709.94; 65%) are obtained.

All the compound detailed hereinafter can be prepared by these general methods for preparing compound both hydroxylated on the nitrogen and non-hydroxylated.

Examples of two-chain glycerophospho-N,N-dimethyl-N-dihydroxypropylalkylammonium compounds

1) 1,2-Dipalmitoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=2) (R₁, R₂═CO—OCH₂)₁₄—CH₃) C₄₂H₈₄NO₁₀P (794.10)

2) 1,2-Distearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=2) (R₁, R₂═CO—(CH₂)₁₆—CH₃) C₄₆H₉₂NO₁₀P (850.20)

3) 1,2-Dimyristoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=2) (R₁, R₂═CO—(CH₂)₁₂—CH₃) C₃₈H₇₆NO₁₀P (737.99)

4) 1,2-Dierucyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=2) (R₁, R₂═CO—(CH₂)₁₁—CH═CH—(CH₂)₇—CH₃) C₅₄H₁₀₄NO₁₀P (958.39)

5) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=2) (R₁, R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₄₆H₈₈NO₁₀P (846.17)

6) 1-Stearoyl-2-oleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=2) (R₁═CO—(CH₂)₁₆—CH₃; R₂═CO—(CH₂)₁₇—CH═CH—(CH₂)₂—CH₃) C₄₆H₉₀NO₁₀P (848.19)

7) 1-Stearoyl-2-myristoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=2) (R₁═CO—(CH₂)₁₆—CH₃; R₂═CO—(CH₂)₁₂—CH₃) C₄₂H₈₄NO₁₀P (794.10)

8) 1-Stearoyl-2-lauroyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=2) (R₁, ═CO—(CH₂)₁₆—CH₃; R₂═CO—(CH₂)₁₀—CH₂) C₄₀H₈₀NO₁₀P (766.04)

9) 1-Lauroyl-2-stearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=2) (R₁—CO—(CH₂)₁₀—CH₃; R₂═CO—(CH₂)₁₆—CH₃) C₄₀H₈₀NO₁₀P (766.04)

10) 1-Erucoyl-2-oleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=2) (R₁═CO—(CH₂)₁₁—CH═CH—(CH₂)₇—CH₃) (R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₅₀H₉₆NO₁₀P (902.28)

11) 1-Oleoyl-2-erucyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=2) (R₁═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) (R₂═CO—(CH₂)₁₁—CH═CH—(CH₂)₇—CH₃) C₅₀H₉₈NO₁₀P (902.28)

12) 1,2-Dipalmitoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=3) (R₁, R₂═Cl—(CH₂)₁₄—CH₃) C₄₃H₈₆NO₁₀P (808.12)

13) 1,2-Dierucyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=3) (R₁, R₂═CO—(CH₂)₁₁—CH═CH—(CH₂)₇—CH₃) C₅₅H₁₀₆NO₁₀P (972.413)

14) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=3) (R₁, R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₄₇H₉₀NO₁₀P (860.20)

15) 1-Stearoyl-2-oleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=3) (R₁═CO—(CH₂)₁₆—CH₃; R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₄₇H₉₂NO₁₀P (862.21)

16) 1-Stearoyl-2-lauroyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=3) (R₁═CO—(CH₂)₁₆—CH₃; R₂═CO—(CH₂)₁₀—CH₃) C₄₁H₈₂NO₁₀P (780.07)

17) 1,2-Dipalmitoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-butylammonium (n=4) (R₁, R₂═CO—(CH₂)₁₄—CH₃) C₄₄H₈₈NO₁₀P (822.15)

18) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-butylammonium (n=4) (R₁, R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₄₈H₉₂NO₁₀P (874.23)

19) 1,2-Dierucyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-butylammonium (n=4) (R₁, R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₅₆H₁₀₈NO₁₀P (986.44)

20) 1-Stearoyl-2-oleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-butylammonium (n=4) (R₁═CO—(CH₂)₁₆—CH₃; R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₄₈H₃₄NO₁₀P (876.24)

21) 1-Stearoyl-2-lauroyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-butylammonium (n=4) (R₁═CO—(CH₂)₁₆—CH₃; R₂═CO—(CH₂)₁₀—CH₃) C₄₂H₈₄NO₁₀P (794.10)

22) 1,2-Dipalmitoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-hexylammonium (n=6) R₁, R₂═CO—(CH₂)₁₄—CH₃) C₄₂, H₈₄NO₁₀P (794.10)

23) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-hexylammonium (n=6) (R₁, R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—(CH₃) C₅₀H₉₆NO₁₀P (902.28)

24) 1,2-Dierucyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-hexylammonium (n=6) (R₁, R₂═CO—(CH₂)₁₁—CH═CH—(CH₂)₇—CH₃) C₅₈H₁₁₂NO₁₀P (1014.49)

Examples of Two-Chain Glycerophospho-N,N-Dimethyl-N-(2-Hydroxypropyl-3,1-O,O-Dihydroxypropyl)Alkylammonium Compounds

25) 1,2-Dipalmitoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1O,O-dihydroxy-propyl)ethylammonium (n=2) (R₁; R₂═CO—(CH₂)₁₄—CH₃) C₄₅H₉₀NO₁₂P (868.18)

26) 1,2-Distearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxy-propyl)ethylammonium (n=2) (R₁; R₂═CO—(CH₂)₁₆—CH₃) C₄₉H₉₈NO₁₂P (924.28)

27) 1,2-Dierucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxypropyl)-ethylammonium (n=2) (R₁; R₂═CO—(CH₂)₁₁—CH═CH—(CH₂)₇—CH₃) C₅₇H₁₁₀NO₁₂P (1032.47)

28) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxypropyl)ethylammonium (n=2) (R₁; R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₄₉H₉₄NO₁₂P (920.25)

29) 1-Stearoyl-2-oleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxy-propyl)ethylammonium (n=2) (R₂═CO—(CH₂)₁₆—CH₃; R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₄₉H₉₆NO₁₂P (922.27)

30) 1-Stearoyl-2-lauroyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxy-propyl)ethylammonium (n=2) (R₁═CO—(CH₂)₁₆—CH₃; R₂═CO—(CH₂)₁₀—CH₃) C₄₃H₈₆NO₁₂P (840.12)

31) 1-Erucoyl-2-oleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxy-propyl)ethylammonium (n=2) (R₂═CO—(CH₂)₁₁—CH═CH—(CH₂)₇—CH₃; R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₅₃H₁₀₂NO₁₂P (976.36)

32) 1-Oleoyl-2-erucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxy-propyl)ethylammonium (n=2) (R₁═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃; R₂═CO—(CH₂)₁₁—CH═CH—(CH₂)₇—CH₃) C₅₃H₁₀₂NO₁₂P (976.36)

33) 1,2-Dipalmitoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxy-propyl)propylammonium (n=3) (R₁, R₂═CO—(CH₂)₁₄—CH₃) C₄₆H₉₂NO₁₂P (822.20)

34) 1,2-Distearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxy-propyl)propylammonium (n=3) (R₁, R₂═CO—(CH₂)₁₆—CH₃) C₅₀H₁₀₀NO₁₂P (938.31)

35) 1,2-Dierucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxypropyl)propylammonium (n=3) (R₁, R₂═CO—(CH₂)₁₁—CH═CH—(CH₂)₇—CH₃) C₅₈H₁₁₂NO₁₂P (1046.49)

36) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxypropyl)-propylammonium (n=3) (R₁, R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₅₀H₉₆NO₁₂P (934.28)

37) 1-Stearoyl-2-oleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxy-propyl)propylammonium (n=3) (R₁═CO—(CH₂)₁₆—CH₃; R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₅₀H₉₈NO₁₂P (936.29)

38) 1-Stearoyl-2-lauroyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxy-propyl)propylammonium (n=3) (R₁═CO—(CH₂)₁₆—CH₃; R₂═CO—(CH₂)₁₀—CH₃) C₄₄H₈₈NO₁₂P (854.15)

39) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxypropyl)butylammonium (N=4) (R₁, R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₅₁H₉₈NO₂₂P (948.30)

40) 1,2-Dierucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxypropyl)butylammonium (n=4) (R₁, R₂═CO—(CH₂)₁₁—CH═CH—(CH₂)₇—CH₃) C₅₉H₁₁₄NO₁₂P (1060.52)

41) 1-Stearoyl-2-oleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxypropyl)butylammonium (n=4) (R₁═CO—(CH₂)₁₆—CH₃; R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₅₁H₁₀₀NO₁₂P (950.32)

42) 1-Stearoyl-2-lauroyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxypropyl)butylammonium (n=4) (R₁═CO—(CH₂)₁₆—CH₃; R₂═CO—(CH₂)₁₀—CH₃) C₄₆H₉₀NO₁₂P (868.175)

43) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxypropyl)hexylammonium (n=6) (R₁, R₂═CO—(CH₂)₇—CH═CH—(CH₂)₇—CH₃) C₆₃H₁₀₂NO₁₂P (976.358)

44) 1,2-Dierucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-dihydroxypropyl)hexylammonium (n=6) (R₁, R₂═CO—(CH₂)₁₁—CH═CH—(CH₂)₇—CH₃) C₆₁H₁₁₈NO₁₂P (1088.57)

Examples of Two-Chain Glycerophospho-N,N-Dimethyl-N-(2-hydroxypropyl-3,1-O,O-2-Hydroxypropyl-2,1-O,O-Dihydroxypropyl)alkylammonium Compounds

45) 1,2-Dipalmitoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-2-hydroxypropyl-3,1-dihydroxypropyl)ethylammonium (n=2) (N=(2-hydroxypropyl-3,1-O,O-2-hydroxypropyl-3,1-dihydroxypropyl) is abbreviated in the following text to N—(HP₁-HP₂-diHP₃) C₄₈H₉₆NO₁₄P (942.25)

46) 1,2-Distearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—diHP₃)ethylammonium (n=2) C₅₂H₁₀₄NO₁₄P (998.36)

47) 1,2-Dierucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)ethylammonium (n=2) C₆₀H₁₁₆NO₁₄P (1106.54)

48) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)ethylammonium (n=2) C₅₂H₁₀₀NO₁₄P (994.33)

49) 1-Stearoyl-2-oleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)ethylammonium (n=2) C₅₂H₁₀₂NO₁₄P (996.35)

50) 1-Stearoyl-2-lauroyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)ethylammonium (N=2) C₄₆H₉₂NO₁₄P (914.20)

51) 1,2-Palmitoyl-2-lauroyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)ethylammonium (n=2) C₄₄H₈₈NO₁₄P (886.15)

52) 1,2-Dipalmitoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)propylammonium (n=3) C₄₉H₉₈NO₁₄P (956.28)

53) 1,2-Distearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)propylammonium (n=3) C₅₃H₁₀₆NO₁₄P (1012.39)

54) 1,2-Dierucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)propylammonium (n=3) C₆₁H₁₁₈NO₁₄P (1120.57)

55) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)propylammonium (n=3) C₅₃H₁₀₂NO₁₄P (1008.36)

56) 1-Stearoyl-2oleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)propylammonium (n=3) C₅₃H₁₀₄NO₁₄P (1010.37)

57) 1-Stearoyl-2-lauroyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)propylammonium (n=3) C₄₇H₉₄NO₁₄P (928.23)

58) 1-Palmitoyl-2-lauroyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)propylammonium (n=3) C₄₅H₉₀NO₁₄P (900.17)

59) 1,2-Dipalmitoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)butylammonium (n=4) C₅₀H₁₀₀NO₁₄P (970.31)

60) 1,2-Distearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)butylammonium (n=4) C₅₄H₁₀₈NO₁₄P (1026.41)

61) 1,2-Dierucoyl-sn-glycero-3-phospho-N,N-dimethyl-N—HP₁—HP₂-diHP₃)butylammonium (n=4) C₆₂H₁₂₀NO₁₄P (1134.60)

62) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)butylammonium (n=4) C₅₄H₁₀₄NO₁₄P (1022.38)

63) 1,2-Dipalmitoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)hexylammonium (n=6) C₅₂H₁₀₄NO₁₄P (998.36)

64) 1,2-Distearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)hexylammonium (n=6) C₅₆H₁₁₂NO₁₄P (1054.47)

65) 1,2-Dierucoyl-sn-glcyero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)hexylammonium (n=6) C₆₄H₁₂₄NO₁₄P (1162.65)

66) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)hexylammonium (n=6) C₅₆H₁₀₈NO₁₄P (1050.44)

67) 1,2-Distearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)octylammonium (N=8) C₅₈H₁₁₆NO₁₄P (1082.52)

68) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)octylammonium (n=8) C₅₈H₁₁₂NO₁₄P (1078.49)

69) 1,2-Dierucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂-diHP₃)octylammonium (n=8) C₆₆H₁₂₈NO₁₄P (1190.70)

Examples of Two-Chain Glycerophospho-N,N-Dimethyl-N-(2-Hydroxypropyl-3,1-O,O-2-Hydroxypropyl-3,1-O,O-2-Hydroxypropyl-3,1-O,O-Dihydroxypropyl)Alkylammonium Compounds

(A=a: n=2,3; R₃, O₃; m=1; x=0, y=1; z=4)

70) 1,2-Dipalmitoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-2-hydroxypropyl-3,1-O,O-hydroxypropyl-3,1-dihydroxypropyl)ethylammonium (n=2) [N-[2-hydroxypropyl-3,1-O,O-2-hydroxypropyl-3,1-O,O-dihydroxypropyl-3,1-O,O-dihydroxypropyl] is abbreviated in the following text to N-(HP₁—HP₂—HP₃-diHP₄). C₉₂H₁₀₂NO₁₆P (1016.33)

71) 1,2-Distearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—HP₃-diHP₄)ethylammonium (n=2) C₅₅H₁₁₀NO₁₆P (1072.44)

72) 1,2-Dierucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—HP₃-diHP₄)ethylammonium (n=2) C₆₃H₁₂₂NO₁₆P (1180.62)

73) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—HP₃-diHP₄)ethylammonium (n=2) C₅₅H₁₀₆NO₁₆P (1068.41)

74) 1-Stearoyl-2-oleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—HP₃-diHP₄)ethylammonium (n=2) C₅₅H₁₀₈NO₁₆P (1072.42)

75) 1-Stearoyl-2-lauroyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—HP₃-diHP₄)ethylammonium (n=2) C₄₉H₉₈NO₁₆P

76) 1-Palmitoyl-2-lauroyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—HP₃-diHP₄)ethylammonium (n=2) C₄₇H₉₄NO₁₆P (960.23)

77) 1,2-Dipalmitoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—HP₃-diHP₄)propylammonium (n=3) C₅₂H₁₀₄NO₁₆P (1030.36)

78) 1,2-Distearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—HP₃-diHP₄)propylammonium (n=3) C₅₆H₁₁₂NO₁₆P (1086.47)

79) 1,2-Dierucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—HP₃-diHP₄)propylammonium (n=3)

80) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—HP₃-diHP₄)propylammonium (n=3) C₅₆H₁₀₈NO₁₆P (1082.43)

81) 1-Stearoyl-2-lauroyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—HP₃-diHP₄)propylammonium (n=3) C₅₀H₁₀₀NO₁₆P (1002.31)

82) 1-Stearoyl-2-oleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—HP₃-diHP₄)propylammonium (n=3) C₅₆H₁₁₀NO₁₆P (1084.45)

83) 1-Arachinoyl-2-oleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—HP₃-diHP₄)propylammonium (n=3) C₅₈H₁₁₄NO₁₆P (1112.50)

84)1-Behenoyl-2-oleoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁—HP₂—HP₃-diHP₄)propylammonium (n=3) C₆₀H₁₁₈NO₁₆P (1140.56)

Examples of Two-Chain Glycerophospho Compounds not Hydroxylated on the Nitrogen

85) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N,N-trimethyl-propylammonium C₄₅H₈₆NO₈P (800.15)

86) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N,N-trimethyl-butylammonium C₄₆H₈₈NO₈P (814.17)

87) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N,N-trimethyl-pentylammonium C₄₇H₉₀NO₈P (828.20)

88) 1,2-Dioleoyl-sn-glycero-3-phospho-N,N,N-trimethyl-hexylammonium C₄₈H₉₂NO₈P (842.23)

89) 1-Stearoyl-2-oleoyl-sn-glycero-3-phospho-N,N,N-trimethylpropylammonium C₄₅H₈₈NO₈P (802.16)

90) 1-Stearoyl-2-oleoyl-sn-glycero-3-phospho-N,N,N-trimethylbutylammonium C₄₆H₉₀NO₈P (816.19)

91) 1-Palmitoyl-2-lauroyl-sn-glycero-3-phospho-N,N,N-trimethylpropylammonium C₃₇H₇₄NO₈P (691.71)

92) 1-Oleoyl-2-lauroyl-sn-glycero-3-phospho-N,N,N-trimethylpropylammonium C₃₉H₇₆NO₈P (718.00)

93) 1-Erucoyl-2-oleoyl-sn-glycero-3-phospho-N,N,N-trimethylpropylammonium C₄₉H₉₄NO_(P ()856.26)

94) 1-Erucoyl-2-oleoyl-sn-glycero-3-phospho-N,N,N-trimethylbutylammonium C₅₀H₉₆NO₈P (870.28)

95) 1-Erucoyl-2-oleoyl-sn-glycero-3-phospho-N,N,N-trimethylhexylammonium C₅₂H₁₀₀NO₈P (898.34)

96) 1Nervonoyl-2-lauroyl-sn-glycero-3-phospho-N,N,N-trimethylpropylammonium C₄₅H₈₈NO_(P ()802.16)

97) 1-Nervonoyl-2-oleoyl-sn-glycero-3-phospho-N,N,N-trimethylpropylammonium C₅₂H₉₈NO₈P (884.31)

2) Solubilizers

It has emerged that certain substances from the compounds presented herein, especially those which are soluble in ethanol, are excellent solubilizers for substances of low solubility in water. Thus, for example, Taxol can be converted in a simple manner into a form which can be administered intravenously. Likewise, for example Taxotere, cyclosporin, cholesterol and derivatives thereof, steroids, cortisone and analogues, erucylphosphocholine (dissolving of the gel-like structures) have proved to be readily soluble.

In particular, substances which have proved useful for this are those having a distance of 3 C atoms between phosphate and ammonium (n=3 in the general formula I), for example the substances from Examples 14, 85, 111, 139, 144, 176. Outstandingly suitable for these purposes is a simple substance 1-erucoyl-1,3-propane-diol-phospho-N,N,N-trimethylpropylammonium (176). This substance can be prepared simply and in high yields on the tonnage scale.

The solubilizers are preferably single-chain compounds, that is to say when A=a one of R₁ and R₂ is hydrogen or an alkyl with 1 to 3 C atoms.

Taxol for intravenous administration

A solution is prepared form 0.3 g of Taxol and 1.75 g of substance No. 176 in 7.95 g of absolute ethanol. The solution is sterilized by filtration and stored at 4° C. until used.

For intravenous administration, the stock solution is diluted 1:10 or 1:100 with physiological saline.

Examples of Single-Chain Glycerophospho-N,N-Dimethyl-N-Dihydroxypropylalkylammonium Compounds

98) 1-Palmitoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-ethylammonium (n=2) C₂₆H₅₄NO_(P ()555.69)

99) 1-Stearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-ethylammonium (n=2) C₂₈H₅₈NO₉P (583.74)

100) 1-Arachinoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-ethylammonium (n=z) C₃₀H₆₂NO₉P (611.79)

101) 1-Behenoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-ethylammonium (n=2) C₃₂H₆₆NO₉P (639.85)

102) 1-Eucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-ethylammonium (n=2) C₃₂H₆₄NO₉P (637.83)

103) 1-Nervonoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-ethylammonium (n=2) C₃₄H₆₈NO₉P (655.88)

104) 1-0-Hexadecyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-ethylammonium (n=2)

105) 1-0-Octadecyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-ethylammonium (n=2) C₂₈H₆₀NO₈P (569.76)

106) 1-0-Eicosanyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-ethylammonium (n=2) C₃₀H₆₄NO₈P (597.81)

107) 1-0-Behenyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-ethylammonium (n=2) C₃₂H₆₈NO₈P (625.86)

108) 1-Palmitoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-propylammonium (n=3) C₂₇H₅₆NO₉P (569.71)

109) 1-Stearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-propylammonium (n=3) C₂₉H₆₀NO₉P (597.77)

110) 1-Behenoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-propylammonium (n=3) C₃₃H₆₈NO₉P (653.87)

111) 1-Erucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-propylammonium (n=3) C₃₃H₆₆NO₉P (651.86)

112) 1-Nervonoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-propylammonium (n=3) C₃₅H₇₀NO₉P (679.91)

113) 1-Stearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-butylammonium (n=4) C₃₀H₆₂NO₃P (611.79)

114) 1-Erucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-butylammonium (n=4) C₃₄H₆₈NO₉P (665.88)

115) 1-Stearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-hexylammonium (n=6) C₃₂H₆₆NO₉P (639.85)

116) 1-Erucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(diHP)-hexylammonium (n=6) C₃₆H₇₂NO₈P (693.94)

Examples of Single-chain Glycerophospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropyl)alklammonium compounds

117) 1-Stearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-ethylammonium (n=2) C₃₁H₆₄NO₁₁P (657.82)

118) 1-Arachinoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-ethylammonium (n=2) C₃₃H₆₆NO₁₁P (685.87)

119) 1-Erucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-ethylammonium (n=2) C₂₅H₇₀NO₁₁P (711.91)

120) 1-Nervonoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-ethylammonium (n=2) C₃₇H₇₄NO₁₁P (739.96)

121) 1-0-Octadecyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-ethylammonium (n=2) C₃₁H₆₆NO₁₀P (643.83)

122) 1-0-Behenyl-sn-glycero-3-phospho-N,N-dimethyl- N-(HP₁-diHP₂)-ethylammonium (n=2) C₃₅H₇₄NO₁₀P (699.94)

123) 1-Stearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-propylammonium (n=3) C₃₂H₆₆NO₁₁P (671.84)

124) 1-Erucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-propylammonium (n=3) C₃₆H₇₂NO₁₁P (725.94)

125) 1-Erucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-butylammonium (n=4) C₃₇H₇₄NO₁₁P (739.98)

126) 1-Erucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-hexylammonium (n=6) C₃₉H₇₈NO₁₁P (768.04)

127) 1-Nervonoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-propylammonium (n=3) C₃₈H₇₆NO₁₁P (754.01)

128) 1-Nervonoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-butylammonium (n=4) C₃₉H₇₈NO₁₁P (768.04)

Examples of Single-chain Glycerophospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-2-hydroxypropyl-3,1-0,0-dihydroxypropyl)alkylammonium compounds

129) 1-Stearoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-ethylammonium (n=2) C₃₄H₇₀NO₁₃P (731.90)

130) 1-Erucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-ethylammonium (n=2) C₃₈H₇₆NO₁₃P (785.99)

131) 1-Nervonoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-ethylammonium (n=2) C₄₀H₈₀NO₁₃P (814.04)

132) 1-Erucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-propylammonium (n=3) C₃₅H₇₈NO₁₃P (800.01)

133) 1-Nervonoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-propylammonium (n=3) C₄₁H₈₂NO₁₃P (828.07)

134) 1-Erucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-butylammonium (n=4) C₄₀H₈₀NO₁₃P (814.04)

135) 1-Erucoyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-hexylammonium (n=6) C₄₂H₈₄NO₁₃P (842.09)

Examples of Single-chain Glycerophospho Compounds Not Hydroxylated on the Nitrogen

136) 1-Palmitoyl-sn-glycero-3-phospho-N,N,N-trimethylpropylammonium (n=3) C₂₅H₅₂NO₇P (509.66)

137) 1-Stearoyl-sn-glycero-3-phospho-N,N,N-trimethylpropylammonium (n=3) C₂₇H₅₆NO₇P (537.71)

138) 1-Behenoyl-sn-glycero-3-phospho-N,N,N-trimethylpropylammonium (n=3) C₃₁H₆₄NO₂P (593.82)

139) 1-Erucoyl-sn-glycero-3-phospho-N,N,N-trimethylpropylammonium (n=3) C₃₁H₆₂NO₇P (591.81)

140) 1-Nervonoyl-sn-glycero-3-phospho-N,N,N-trimethylpropylammonium (n=3) C₃₃H₅₆NO₇P (619.86)

Examples of ω,ω′-Alkanediol-Phospho-N,N-Dimethyl-N-di-Hydroxypropylalkylammonium Compounds

141) 1-Stearoyl-ethylene glycol-phospho-N,N-dimethyl-N-dihydroxypropylethylammonium (n=2) C₂₇H₅₆NO₈P (553.71)

142) 1-Behenoyl-1,3-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylethylammonium (n=2) C₃₂H₆₆NO₆P (623.85)

143) 1-Stearoyl-1,3-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylethylammonium (n=2) C₂₈H₅₈NO₈P (567.74)

144) 1-Erucoyl-1,3-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylethylammonium (n=2) C₃₂H₆₄NO₈P (621.83)

145) 1-Erucoyl-1,3-propanediol-phospho-N,N-dimethyl-N-dihydroxyproylpropylammonium (n=3) C₃₃H₆₆NO₈P (635.86)

146) 1-Erucoyl-1,3-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylbutylammonium (n=4) C₃₄H₆₈NO₈P (649.88)

Examples of 1,2-Alkanediol-Phospho-N,N-Dimethyl-N-di-Hydroxypropylalkylammonium Compounds

147) 2-Erucoyl-1,2-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylethylammonium C₃₂H₆₄NO₈P (621.33)

148) 1-Erucoyl-1,2-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylethylammonium C₃₂H₆₄NO₈P (621.33)

149) 2-Erucoyl-1,2-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylpropylammonium C₃₃H₆₆NO₈P (635.86)

150) 1-Erucoyl-1,2-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylbutylammonium C₃₄H₆₈NO₈P (649.88)

Examples of ω,ω′-Alkanediol-Phospho-N,N-Dimethyl-N-(2-Hydroxypropyl)-3,1-0,0-Dihydroxypropyl)Alkyl-Ammonium Compounds

151) 1-Stearoyl-ethylene glycol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropyl)ethylammonium C₃₀H₆₂NO₁₀ _(P ()627.79)

152) 1-Behenoyl-1,3-propanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropyl)ethylammonium C₃₅H₇₂NO₁₀P (697.93)

153) 1-Stearoyl-1,3-propanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropylethylammonium C₃₁H₆₄NO₁₀P (641.82)

154) 1-Erucoyl-1,3-propanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropyl)ethylammonium C₃₅H₇₀NO₁₀P (695.91)

155) 1-Erucoyl-1,3-propanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropyl)propylammonium C₃₆H₇₂NO₁₀P (709.94)

156) 1-Erucoyl-1,3-propanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropyl)butylammonium C₃₇H₇₄NO₁₀P (723.96)

157) 1-Erucoyl-1,4-butanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropyl)propylammonium C₃₇H₇₄NO₁₀P (723.96)

158) 1-Erucoyl-1,6-hexanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropyl)propylammonium C₃₉H₇₈NO₁₀P (752.02)

159(1-Erucoyl-1,8-octanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl)-3,1-0,0-dihydroxypropyl)propylammonium C₄₁H₈₂NO₁₀P (780.07)

Examples of 1,2-Alkanediol-Phospho-N,N-Dimethyl-N-(2-Hydroxypropyl-3,1-0,0-Dihydroxypropyl)Alkylammonium Compounds

160) 2-Erucoyl-1,2-propanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropyl)ethylammonium C₃₅H₇₀NO₁₀P (695.91)

161) 1-Erucoyl-1,2-propanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropyl)ethylammonium C₃₅H₇₀NO₁₀P (695.91)

162) 2-Erucoyl-1,2-propanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0, dihydroxypropyl)propylammonium C₃₆H₇₂NO₁₀P (709.94)

163) 1-Erucoyl-1,2-propanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropyl)butylammonium C₃₇H₇₄NO₁₀P (723.96)

164) 1-Erucoyl-1,2-butanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropyl)propylammonium C₃₇H₇₄NO₁₀P (723.96)

165) 1-Erucoyl-1,2-hexanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropyl)propylammonium C₃₉H₇₈NO₁₀P (752.02)

166) 1-Erucoyl-1,2-octanediol-phospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-0,0-dihydroxypropyl)propylammonium C₄₁H₈₂NO₁₀P (780.07)

Examples of ω,ω′-Alkanediol-N,N-Dimethyl-N-(2-Hydroxy-Propyl-3,1-0,0-2-hydroxypropyl-3,1-0,0-dihydroxypropyl)Alkylammonium Compounds

167) 1-Oleoyl-ethylene glycol-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-ethylammonium C₃₄H₆₈NO₁₂P (713.88)

168) 1-Erucoyl-1,3-propanediol-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-ethylammonium C₃₈H₇₆NO₁₂P (769.99)

169) 1-Oleoyl-1,3-propanediol-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-propylammonium C₃₅H₇₀NO₁₂P (727.91)

170) 1-Erucoyl-1,3-propanediol-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-propylammonium C₃₉H₇₈NO₁₂P (784.01)

171) 1-Erucoyl-1,3-propanediol-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-butylammonium C₄₀H₈₀NO₁₂P (798.04)

172) 1-Erucoyl-1,3-propanediol-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-hexylammonium C₄₂H₈₄NO₁₂P (826.10)

Examples of Alkanediol-phospho Compounds Not Hydroxylated on the Nitrogen

173) 1-Erucoyl-ethylene glycol-phospho-N,N,N-trimethylpropylammonium C₃₀H₆₀NO₆P (561.78)

174) 1-Arachinoyl-1,3-propanediol-phospho-N,N,N-trimethylpropylammonium C₂₉H₆₉NO₆P (549.77)

175) 1-Stearoyl-1,3-propanediol-phospho-N,N,N-trimethylpropylammonium C₂₇H₅₆NO₆P (521.71)

176) 1-Erucoyl-1,3-propanediol-phospho-N,N,N-trimethylpropylammonium C₃₁H₆₂NO₆P (575.81)

177) 1-Erucoyl-1,3-propanediol-phospho-N,N,N-trimethylbutylammonium C₃₂H₆₄NO₆P (589.83)

178) 1-Erucoyl-1,3-propanediol-phospho-N,N,N-trimethylpentylammonium C₃₃H₆₆NO₆(603.86)

179) 1-Erucoyl-1,2-propanediol-phospho-N,N,N-trimethylpropylammonium C₃₁H₆₂NO₆P (575.81)

180) 2-Erucoyl-1,2-propanediol-phospho-N,N,N-trimethylpropylammonium C₃₁H₆₂NO₆P (575.81)

181) 1-Erucoyl-1,2-propanediol-phospho-N,N,N-trimethylbutylammonium C₃₂H₆₄NO₆P (589.83)

182) 1-Erucoyl-1,2-propanediol-phospho-N,N,N-trimetylhexylammonium C₃₄H₆₈NO₆P (617.92)

3) Active ingredients

It was found in earlier investigations that alkylphosphocholines have antitumour activity only when the phosphate-ammonium distance is two C atoms, that is to say corresponds to phosphocholine (n=2 in the general formula I). Compounds with a distance of n>2 had no activity. The active ingredients were administered orally in these earlier investigations.

We have now found, surprisingly, that erucylphospho compounds with phosphate-ammonium distances of >2 have excellent antitumour activity which is in fact superior to that of alkylphosphocholines when these substances are administered intravenously, as the following comparison shows:

Erucylphosphocholine (n=2 in general formula I)

The substance forms gel-like structures in water and therefore can be administered intravenously only with difficulty in relatively high concentrations. Erucylphosphocholine has only a slight long-term effect in the animal model of methylnitrosourea-induced mammary carcinoma. Tumour growth is observed again only 7 days after discontinuation of the therapy.

Erucylphospho-N,N,N-trimethylpropylammonium (n=3)

The substance is readily soluble in water, forms no gels and can be administered intravenously without difficulty. It can therefore also be used as solubilizer. However, its long-term effect in the above animal model is particularly noteworthy and impressive. No new tumour growth is observed even 4 weeks after discontinuation of the therapy.

Examples of Alkylphospho-N,N,N-Dimethyl-N-Dihydroxy-Propylalkylammonium Compounds

183) Hexadecylphospho-N,N-dimethyl-N-dihydroxypropylethylammonium C₂₃H₅₀NO₆P (467.62)

184) Octadecylphospho-N,N-dimethyl-N-dihydroxypropylethylammonium C₂₅H₅₄NO₆P (495.68)

185) Erucylphospho-N,N-dimethyl-N-dihydroxypropylethylammonium C₂₉H₆₀NO₆P (549.77)

186) Erucylphospho-N,N-dimethyl-N-dihydroxypropylpropylammonium C₃₀H₆₂NO₆P (563.80)

187) Erucylphospho-N,N-dimethyl-N-dihydroxypropylbutylammonium C₃₁H₆₄NO₆P (577.82)

188) Erucylphospho-N,N-dimethyl-N-dihydroxypropylhexylammonium C₃₃H₆₈NO₆P (605.88)

189) Oleylphospho-N,N-dimethyl-N-dihydroxypropylethylammonium C₂₅H₅₂NO₆P (493.66)

190) Oleylphospho-N,N-dimethyl-N-dihydroxypropylpropylammonium C₂₆H₅₄NO₆P (507.69)

191) Oleylphospho-N,N-dimethyl-N-dihydroxypropylbutylammonium C₂₇H₅₆NO₆P (521.21)

192) (Z-11)-Eicosenylphospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium C₂₇H₅₆NO₆P (521.21)

193) (Z-11)-Eicosenylphospho-N,N-dimethyl-N-dihydroxypropyl-propylammonium C₂₈H₅₈NO₆P (535.74)

194) (Z-11)-Eicosenylphospho-N,N-dimethyl-N-dihydroxypropyl-butylammonium C₂₉H₆₀NO₆P (549.77)

195) (Z-11)-Eicosenylphospho-N,N-dimethyl-N-dihydroxypropyl-pentylammonium C₃₀H₆₂NO₆P (563.80)

196) (Z-11)-Eicosenylphospho-N,N-dimethyl-N-dihydroxypropyl-hexylammonium C₃₁H₆₄NO₆P (577.82)

Examples of Alkylphospho-N,N-Dimethyl-N-(2-Hydroxypropyl-1,2-Dihydroxypropyl)Alkylammonium

197) Hexadecyl-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-ethylammonium C₂₆H₅₆NO₈P (541.70)

198) Octadecyl-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-ethylammonium C₂₈H₆₀NO₈P (569.76)

199) Erucyl-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-ethylammonium C₃₂H₆₆NO₈P (623.85)

200) Erucyl-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-propylammonium C₃₃H₆₈NO₈P (637.87)

201) Erucyl-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-butylammonium C₃₄H₇₀NO₈P (651.90)

202) Erucyl-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-hexylammonium C₃₆H₇₄NO₈P (679.95)

203) Oleyl-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-ethylammonium C₂₈H₅₈NO₈P (567.74)

204) Oleyl-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-propylammonium C₂₉H₆₀NO₈P (581.77)

205) Oleyl-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-butylammonium C₃₀H₆₂NO₈P (595.79)

206) (Z-11)-Eicosenyl-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-ethylammonium C₃₀H₆₂NO₈P (595.79)

207) (Z-11)-Eicosenyl-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-propylammonium C₃₀H₆₄NO₆P (609.82)

208) (Z-11)-Eicosenyl-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-butylammonium C₃₂H₆₆NO₈P (623.85)

Examples of alkylphospho-N,N-dimethyl-N-(2-hydroxypropyl-3,1-O,O-hydroxypropyl-3,1-O,O-1,2-dihydroxypropyl)alkylammonium compounds

209) Hexadecyl-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-ethylammonium C₂₉H₆₂NO₁₀P (615.78)

210) Octadecyl-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-ethylammonium C₃₁H₆₆NO₁₀P (643.83)

211) Oleol-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-ethylammonium C₃₁N₆₄NO₁₀P (641.82)

212) (Z-11)-Eicosenyl-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-ethylammonium C₃₃H₆₈NO₁₀P (669.87)

213) (Z-11)-Eicosenyl-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-propylammonium C₃₄H₇₀NO₁₀P (683.90)

214) (Z-11)-Eicosenyl-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-butylammonium C₃₅H₇₁NO₁₀P (697.93)

215) Erucyl-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-ethylammonium C₃₅H₇₂NO₁₀P (697.93)

216) Erucyl-phospho-N,N-dimethyl-N-(HP₁-HP₂-diHP₃)-propylammonium C₃₆H₇₄NO₁₀P (711.95)

Examples of Alkylphospho Compounds Having no Dihydroxyalkyl Radicals on the Nitrogen

217) Erucyl-phospho-N,N,N-trimethyl-propylammonium C₂₈H₅₈NO₄P (503.74)

218) Erucyl-phospho-N,N-dimethyl-N-ethyl-propylammonium C₂₉H₆₀NO₄P (517.77)

219) Erucyl-phospho-N,N-dimethyl-N-propyl-propylammonium C₃₀H₆₂NO₄P (531.80)

220) Erucyl-phospho-N,N-dimethyl-N-allyl-propylammonium C₃₀H₆₀NO₄P (529.78)

Rf values of substances 217-240 in the system described: Rf 0.30-0.40

221) (Z)-10-Docosenyl-2-phospho-N,N,N-trimethyl-propylammonium C₂₈H₅₈NO₄P (503.74)

222) (Z)-10-Docosenyl-2-phospho-N,N-dimethyl-N-ethyl-propylammonium C₂₉H₆₀NO₄P (517.77)

223) Erucyl-phospho-N,N,N-trimethyl-butylammonium C₂₉H₆₀NO₄P (517.77)

224) Erucyl-phospho-N,N-dimethyl-N-ethyl-butylammonium C₃₀H₆₂NO₄P (531.80)

225) Erucyl-phospho-N,N-dimethyl-N-propyl-butylammonium C₃₁H₆₄NO₄P (545.82)

226) (Z)-10-Docosenyl-2-phospho-N,N,N-trimethyl-butylammonium C₂₉H₆₀NO₄P (517.77)

227) (Z)-11-Eicosenyl-phospho-N,N,N-trimethyl-propylammonium C₂₆H₅₄NO₄P (475.69)

228) (Z)-11-Eicosenyl-phospho-N,N-dimethyl-N-ethyl-propylammonium C₂₇H₅₆NO₄P (489.72)

229) (Z)-11-Eicosenyl-phospho-N,N-diethyl-N-methyl-propylammonium C₂₈H₅₈NO₄P (503.74)

230) (Z)-11-Eicosenyl-phospho-N,N-dimethyl-N-propyl-propylammonium C₂₈H₅₈NO₄P (503.74)

231) (Z)-11-Eicosenyl-phospho-N,N,N-trimethyl-butylammonium C₂₇H₅₆NO₄P (489.72)

232) (Z)-11-Eicosenyl-phospho-N,N-dimethyl-N-ethyl-butylammonium C₂₈H₅₈NO₄P (503.74)

233) (Z)-11-Eicosenyl-phospho-N,N-dimethyl-N-propyl-butylammonium C₂₉H₆₀NO₄P (517.77)

234) (Z)-11-Eicosenyl-phospho-N,N-dimethyl-N-alkyl-butylammonium C₂₉ ₅₈NO₄P (575.75)

235) Oleyl-phospho-N,N,N-trimethyl-propylammonium C₂₄H₅₀NO₄P (447.64)

236) Oleyl-phospho-N,N-dimethyl-N-ethyl-propylammonium C₂₅H₅₂NO₄P (461.66)

237) Oleyl-phospho-N,N-dimethyl-N-propylene-propylammonium C₂₆H₅₄NO₄P (475.69)

238) Oleyl-phospho-N,N,N-trimethyl-butylammonium C₂₅H₅₂NO₄P (461.66)

239) Oleyl-phospho-N,N-dimethyl-N-ethyl-butylammonium C₂₆H₅₄NO₄P (475.69)

240) Oleyl-phospho-N,N-dimethyl-N-propyl-butylammonium C₂₇H₅₆NO₄P (489.72)

Active Ingredients Based on Alkylated (Ether)Lysole-Cithins and Hydroxylated on the Nitrogen

241) 1-0-Octadecyl-2-0-methyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=2) C₂₉H₆₂NO₈P (583.78)

242) 3-0-Octadecyl-2-0-methyl-sn-glycero-1-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=2) C₂₉H₆₂NO₈P (583.78)

243) 1-0-Octadecyl-2-0-tert-butyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydrxoypropyl-ethylammonium (n=2) C₃₂H₆₈NO₈P (625.86)

244) 3-0-Octadecyl-2-0-tert-butyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-ethylammonium (n=2) C₃₂H₆₈NO₈P (625.86)

245) 1-0-Octadecyl-2-0-methyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-propylammonium (n=3) C₃₀H₆₄NO₈P (597.81)

246) 1-0-Octadecyl-2-0-methyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-butylammonium (n=4) C₃₁H₆₆NO₈P (611.84)

247) 1-0-Erucyl-2-0-methyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxpropyl-ethylammonium (n=2) C₃₃H₆₈NO₈P (637.87)

248) 1-0-Erucyl-2-0-methyl-sn-glycero-3-phospho-N,N-dimethyl-N-dihydroxypropyl-propylammonium (n=3) C₃₄H₇₀NO₈P (651.90)

249) 1-0-Octadecyl-2-0-methyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-ethylammonium (n=2) C₃₂H₆₈NO₁₀P (657.86)

250) 1-0-Ocetadecyl-2-0-tert-butyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-ethylammonium (n=2) C₃₅H₇₄NO₁₀P (699.94)

251) 1-0-Ocetadecyl-2-0-methyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-propylammonium (n=3) C₃₃H₇₀NO₁₀P (671.89)

252) 1-0- Octadecyl-2-0-tert-butyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-propylammonium (n=3) C₃₆H₇₆NO₁₀P (713.97)

253) 1-0-Octadecyl-2-0-tert-butyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-butylammonium (n=4) C₃₇H₇₈NO₁₀P (727.99)

254) 1-0-Erucyl-2-0-methyl-sn-glycero-3-phospho-N,N-dimethyl-N-(HP₁-diHP₂)-butylammonium (n=4) C₃₈H₇₈NO₁₀P (739.01)

Active Ingredient Based on Alkylated (Ether)Lysole-Cithins and not Hydroxylated on the Nitrogen

255) 1-0-Erucyl-2-0-methyl-sn-glycero-3-phospho-N,N,N-trimethyl-propylammonium (n=3) C₃₂H₆₆NO₆P (591.85)

256) 1-0-Erucyl-3-0-methyl-sn-glycero-2-phospho-N,N,N-trimethyl-propylammonium (n=3) C₃₂H₆₆NO₆P (591.85)

257) 1-0-(Z)-11-Eicosenyl-2-0-methyl-sn-glycero-3-phospho-N,N,N-trimethyl-propylammonium (n=3) C₃₀H₆₂NO₆P (563.80)

258) 1-0-(Z)-11-Eicosenyl-2-0-tert-butyl-sn-glycero-3-phospho-N,N,N-trimethyl-propylammonium (n=3) C₃₃H₆₈NO₆P (605.88)

259) 1-0-Oleyl-2-0-tert-butyl-sn-glycero-3-phospho-N,N,N-trimethyl-propylammonium (n=3) C₃₁H₆₄NO₆P (577.82)

260) 1-0-(Z)-11-Eicosenyl-2-0-tert-butyl-sn-glycero-3-phospho-N,N,N-butylammonium (n=4) C₃₄H₇₀NO₆P (619.90)

261) 1-0-Oleyl-2-0-tert-butyl-sn-glycero-3-phospho-N,N,N-trimethyl-butylammonium (n=4) C₃₂H₆₆NO₆P (591.85)

Active Ingredients Based on Alkanediol-Phospho Compounds and Hydroxylated on the Nitrogen

262) 1-0-Erucyl(ethylene glycol)phospho-N,N-dimethyl-N-dihydroxypropylethylammonium C₃₁H₆₄NO₇P (593.82)

263) 1-0-Erucyl-1,3-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylethylammonium C₃₂H₆₆NO₇P (607.85)

264) 1-0-Erucyl-1,2-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylethylammonium C₃₂H₆₆NO₇P (607.85)

265) 2-0-Erucyl-1,2-propanediol-phospho-N,N-dimethyl-N-dihydroxpropylethylammonium C₃₂H₆₆NO₇P (607.85)

266) 1-0-(Z)-11-Eicosenyl-1,2-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylethylammonium C₃₀H₆₂NO₇P (579.49)

267) 2-0-(Z)-11-Eicosenyl-1,2-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylethylammonium C₃₀H₆₂NO₇P (579.49)

268) 1-0-Oleyl-1,2-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylethylammonium C₂₈H₅₈NO₇P (551.74)

269) 2-0-Oleyl-1,2-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylethylammonium C₂₈H₅₈NO₇P (551.74)

270) 2-0-Octadecyl-1,2-propanediol-phospho-N,N-dimethyl-N-dihydroxylpropylethylammonium C₂₈H₆₀NO₇P (553.76)

271) 1-0-Octadecyl-1,2-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylpropylammonium C₂₉H₆₂NO₇P (626.24)

272) 2-0-Octadecyl-1,2-propanediol-phospho-N,N-dimethyl-N-dihydroxypropylpropylammonium C₂₉H₆₂NO₇P (626.24)

Active Ingredients Based on Alkanediol-Phospho Compounds and not Hydroxylated on the Nitrogen

273) 1-0-Erucyl-1,2-propanediol-phospho-N,N,N-trimethylpropylammonium C₃₁H₆₄NO₅P (562.82)

274) 1-0-Erucyl-1,2-propanediol-phospho-N,N,N-trimethylpropylammonium C₃₁H₆₄NO₅P (562.82)

275) 1-0-(Z)-11-Eicosenyl-1,3-propanediol-phospho-N,N,N-trimethylpropylammonium C₂₉H₆₀NO₅P (533.77)

276) 1-0-Oleyl-1,2-propanediol-phospho-N,N,N-trimethylpropylammonium C₂₇H₅₆NO₅P (505.72)

277) 2-0-Oleyl-1,2-propanediol-phospho-N,N,N-trimethylpropylammonium C₂₇H₅₆NO₅P (505.72)

278) 1-0-Octadecyl-1,2-propanediol-phospho-N,N,N-propylammonium C₂₇H₅₈NO₅P (507.73)

279) 2-0-Octadecyl-1,2-propanediol-phospho-N,N,N-trimethylpropylammonium C₂₇H₅₈NO₅P (507.73) 

What is claimed is:
 1. A compound of the general formula (I)

in which A is

or

where R₁ and R₂ are, independently of one another, hydrogen, a saturated or unsaturated acyl or acyl radical which can optionally be branched or/and substituted, where the total of the carbon atoms in the acyl and alkyl is 16 to 44 C atoms, s is an integer from 0 to 8, or (f) a radical of a primary or secondary alcohol of the formula RO—, where R is a saturated or unsaturated alkyl radical, mainly with cis double bond, of from 12 to 30 carbon atoms, n is an integer from 2 to 8, and wherein in general formula (I) R₃ is (aa) 1,2-dihydroxypropyl or (bb) alkyl with 1 to 3 C atoms when z is >0 or (cc) alkyl with 1 to 3 carbon atoms when n≠2 and z=0), x is an integer from 0 to 8, y is 1 for z=1 to 5 or is 1 to 4 for z=1 z is an integer from
 0. 2. Compound according to claim 1 with m=1.
 3. Compound according to claim 2 with m=1 x=0 y=1 z=1.
 4. Compound according to claim 2 with m=1 x=0 y=1 z=2.
 5. Compound according to claim 2 with m=1 x=0 y=1 z=3.
 6. Compound according to claim 2 with m=1 x=0 y=1 z=4.
 7. Compound according to claim 2 with m=1 x=1-3 z=0.
 8. Compound according to claim 1 with n=2-6.
 9. Compound according to any of the preceding claims with n=2-6.
 10. Compound according to claim 1, in which R₃ is a CH₃ radical.
 11. Compound according to claim 1, characterized in that A is a group of the formula a.
 12. Compound according to claim 1, characterized in that A is a group of the formula a, and one of R₁ and R₂ is H or an alkyl chain with 1-3 C atoms.
 13. Compound according to a claim 1, characterized in that A is a group of the formula a, and n=3-6.
 14. Compound according to any of the preceding claims, characterized in that A is a group of the formula a, and n=3-6.
 15. Compound according to claim 14, characterized in that n=3.
 16. Compound according to claim 1, characterized in that A is a group of the formula b.
 17. Compound according to any of claims 1 to 11, characterized in that A is a group of the formula b.
 18. Compound according to claim 1, characterized in that n=3.
 19. Compound according to claim 1, characterized in that n=4.
 20. Compound according to claim 1, characterized in that A is a group of the formula c.
 21. Compound according to any of claims 1 to 11, characterized in that A is a group of the formula c.
 22. Liposomes which contain phospholipids or/and alkyl phospholipids, where a cholesterol and 1-50 mol % of a compound of the general formula (I) of claim 1 or salts thereof, where the cholesterol, the phospholipids, the alkyl phospholipids and the compound of the formula (I) together amount of 100 mol %.
 23. The compound of the general formula (I) of claim 1 wherein the compound is formulated as a gene transport vehicle.
 24. The compound of the general formula (I) of claim 1 wherein the compound is formulated as a solvent for water-insoluble active ingredients.
 25. The compound of claim 24, wherein R₁ is an erucic acid residue.
 26. Use according to claim 25, characterized in that R₁ is an erucic acid residue. 